Cultured cells used in research can be misidentified or can become altered, either by contamination from other cell lines or by spontaneous genetic changes after a high number of subcultures. The American Type Culture Collection (ATCC®) recommends using low passage cell lines and authentication of cell lines used in research to assure reliable and reproducible results. Low passage minimizes the number of spontaneous changes that arise. Authentication provides a means to:
The Molecular Diagnostics Laboratory has adopted an American National Standards Institute (ANSI) recommended protocol to authenticate human cell lines as a service to investigators. The Promega GenePrint® 10 system uses a multiplex PCR approach to coamplify 9 highly polymorphic STR markers and the Amelogenin gene in the human genome. The amplified DNA fragments of each sample are resolved on the Applied Biosystems 3130xl 16-capillary Genetic Analyzer with Applied Biosystems GeneMapper v 4.0 software to determine cell line genotypes.
*Please note that this genotyping method assays 10 human loci in the genome. Changes that may have arisen at other loci during cell culture will not be detected by this method.