OverviewTotal RNA or low molecular weight-enriched RNA is converted into cDNA using a single "megaplex" reverse transcriptase reaction. The megaplex reaction contains a specific stem-loop primer for each of the mature target microRNA's (Each stem-loop primer is designed to hybridize to only the fully mature microRNA, and not to precursor forms of its target.). The cDNA is subjected to a "pre-amplification" PCR. The diluted pre-amplification products serve as substrates for TaqMan® quantitative PCR reactions. The assays that comprise this process are manufactured by Applied Biosystems of Life Technologies, Inc.
TLDA cards - A profiling service is available on the human and rodent pairs of the following TaqMan Low Density Array (TLDA) cards: |
Note: The current Human and Rodent TLDA card sets offered are version 3, comprised of version 2.0A card and version 3.0B card. The content of the version 3.0 card sets are consistent with Sanger miRBase v20. Annotation information can be found here.