Total RNA or low molecular weight-enriched RNA is converted into cDNA using a single "megaplex" reverse transcriptase reaction. The megaplex reaction contains a specific stem-loop primer for each of the mature target microRNA's (Each stem-loop primer is designed to hybridize to only the fully mature microRNA, and not to precursor forms of its target.). The cDNA is subjected to a "pre-amplification" PCR. The diluted pre-amplification products serve as substrates for TaqMan® quantitative PCR reactions. The assays that comprise this process are manufactured by Applied Biosystems of Life Technologies, Inc.
TLDA cards - A profiling service is available on the human and rodent pairs of the following TaqMan Low Density Array (TLDA) cards: