Human Cell Line Identity Verification - Genetic loci
The Promega GenePrint® 10 System coamplifies 10 human loci including 9 polymorphic tetranucleotide short tandem repeats (STR) and the Amelogenin gene for gender identification. The PCR amplicons, each less than 500 bp, are labeled with fluorescein (FL), 6-carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein ( JOE) or carboxy-tetramethylrhodamine (TMR).
Adapted from Promega GenePrint ® 10 System Technical Manual TM392.
The loci selected by Promega for the assay have a high degree of heterozygosity and produce a minimum of artifacts associated with Taq DNA polymerase such as repeat slippage and terminal nucleotide addition. The primers selected for each locus generate amplified products within a limited size range allowing simultaneous detection of several loci without overlap of alleles across loci.
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