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miRNA Expression Profiling - Step 3: Pre-amplification
A portion of the cDNA reaction is added to a "pre-amplification" PCR reaction. The primers in the pre-amplification reaction consist of :
(1) a mixture of forward primers, each of which anneals to a specific miRNA cDNA, and
(2) a single reverse primer sequence that anneals to the complement of the stem/loop primer "tail", a sequence that is present in every miRNA cDNA.
Twelve cycles of PCR are carried out.
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Human Cell Line Identity Verification
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Microsatellite Instability Analysis
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Sample Prep/Submission
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