The investigator is responsible for analyzing and interpreting the data.
Two types of files will be posted to the investigator's password-protected directory, which is accessible from the "Results" section of this website:
(1) One Microsoft Excel workbook for each sample submitted, containing the CT values for each of the microRNA assays. The CT values can be used to determine ΔCT values, ΔΔCT values and RQ (relative quantity) values.
(2) An .sds file for each TLDA card, which is generated by software associated with the 7900HT instrument.
ABI Sequence Detection Software is necessary if you wish to analyze the raw .sds files. Among other functions, the SDS software permits investigators to adjust analysis parameters and to display selected data in a variety of views. Click here for a document which offers some guidance on setting up a relative quantitation experiment, and gives some instruction on using the SDS software.
The assay list spreadsheets found on the miRNA expression profiling page contain columns describing how the assay corresponds to the current miRBase entry, as well as the target sequence. If you click on an entry in one of the description columns (you may also have to press the Return / Enter key), it should call up the miRBase web page for that assay.